Drug Controlled Release and Biological Behavior of Poly(D,L-Lactide-Co-Glycolide) Microspheres

Poly(D,L-lactide-co-glycolide) (PLGA, 75/25) microspheres loaded with bovine serum albumin (BSA) were prepared using the W/O/W emulsification solvent evaporation technique. The cytotoxicity in vitro of PLGA microspheres was investigated and the BSA release from PLGA microspheres was also studied. Scanning electron micrographs showed that the PLGA microspheres were regular and the surface was smooth. BSA release typically began with an initial burst and then became steady. Analysis of the PLGA microspheres cytotoxicity showed that they had no cytotoxic effect and behaved very similar to the negative control of polystyrene. The hemolysis rate of the PLGA microspheres was 0.148%, suggesting it had no potential to induce hemolysis. The results show that PLGA microspheres may provide a useful controlled release protein drug system for used in pharmaceutics.