Real-time quantification of Staphylococcusaureus in liquid medium using infrared thermography |
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Authors: | Ahmad A SalaimehJeffrey J Campion Belal Y Gharaibeh Martin E Evans Kozo Saito |
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Institution: | a Institute of Research for Technology Development (IR4TD), College of Engineering, University of Kentucky, Lexington, KY, United States b Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, University of Kentucky, Lexington, KY, United States c Veterans Affairs Medical Center, Lexington, KY, United States |
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Abstract: | We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichiacoli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcusaureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S.aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 108 CFU/ml. The EC of S.aureus was ∼2-fold higher than that of E.coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera. |
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Keywords: | Infrared thermography Energy content Real-time Bacterial quantification Staphylococcus aureus |
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