Electrochemical polymerization of N-substituted pyrrols for the development of novel lactate biosensor |
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Authors: | L Ch Korneyeva A V Borisova Y I Yashina E E Karyakina O G Voronin S Cosnier A A Karyakin |
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Institution: | (1) Department of Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Boterhoeksestraat 48, 6666 GA Heteren, The Netherlands |
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Abstract: | Lactate oxidase from the species Pediococcus is immobilized in a conducting polymer film on the surface of planar electrodes modified with Prussian blue. Polypyrrole
ammonium is electropolymerized to obtain the conducting polymer. The analytical characteristics of the resulting biosensor
are as follows: a sensitivity of 190 ± 14 mA M−1 cm−2, a linear dynamic range of 5 × 10−7 to 5 × l0−4 M, and high operational stability. The applicability of a lactate biosensor for food quality control (for example, quality
control of kvass) is shown. Effective and inexpensive biosensors for lactate analysis may be applied in clinical diagnostics,
sports medicine, quality control of food and farm products, as well as for biotechnology processes. |
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