Electron transfer dissociation with supplemental activation to differentiate aspartic and isoaspartic residues in doubly charged peptide cations |
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Authors: | Wai Yi Kelly Chan T W Dominic Chan Peter B O’Connor |
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Institution: | 1.Department of Chemistry,The Chinese University of Hong Kong,Hong Kong, China;2.Department of Chemistry,University of Warwick,West Midlands,UK |
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Abstract: | Electron-transfer dissociation (ETD) with supplemental activation of the doubly charged deamidated tryptic digested peptide
ions allows differentiation of isoaspartic acid and aspartic acid residues using the c + 57 or z • − 57 peaks. The diagnostic peak clearly localizes and characterizes the isoaspartic acid residue. Supplemental activation
in ETD of the doubly charged peptide ions involves resonant excitation of the charge reduced precursor radical cations and
leads to further dissociation, including extra backbone cleavages and secondary fragmentation. Supplemental activation is
essential to obtain a high quality ETD spectrum (especially for doubly charged peptide ions) with sequence information. Unfortunately,
the low-resolution of the ion trap mass spectrometer makes detection of the diagnostic peak, M-60], for the aspartic acid
residue difficult due to interference with side-chain loss from arginine and glutamic acid residues. |
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