| NAD-硅胶亲和色谱固定相的合成和在核苷酸及其碱基分析中的应用 |
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| 引用本文： | 于世林,苗凤琴,杨朝霞,冯茹.NAD-硅胶亲和色谱固定相的合成和在核苷酸及其碱基分析中的应用[J].高等学校化学学报,1995,16(8):1212. |
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| 作者姓名： | 于世林 苗凤琴 杨朝霞 冯茹 |
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| 作者单位： | 北京化工大学应用化学系, 北京, 100029 |
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| 摘 要： | ＮＡＤ－硅胶亲和色谱固定相的合成和在核苷酸及其碱基分析中的应用于世林，苗凤琴，杨朝霞，冯茹（北京化工大学应用化学系，北京，１０００２９）关键词烟酰胺腺嘌呤二核苷酸（ＮＡＤ），亲和色谱，核苷酸烟酰胺腺嘌呤二核苷酸（ＮＡＤ，辅酶Ⅰ）是多种脱氢酶的辅酶，通...
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| 关 键 词： | 烟酰胺腺嘌呤二核苷酸(NAD) 亲和色谱 核苷酸 |
| 收稿时间： | 1994-08-18 |
The Application of an NAD-Silica Biospecific Affinity Chromatography Stationary Phase in Analysis of Nucleotides |
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| YU Shi-Lin, MIAO Feng-Qin, YANG Zhao-Xia, FENG Ru.The Application of an NAD-Silica Biospecific Affinity Chromatography Stationary Phase in Analysis of Nucleotides[J].Chemical Research In Chinese Universities,1995,16(8):1212. |
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| Authors: | YU Shi-Lin MIAO Feng-Qin YANG Zhao-Xia FENG Ru |
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| Institution: | Dept.of Appl. Chem., Beijing University of Chemical Technology, 100029 |
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| Abstract: | By using a wide-pore(30 nm),microspheric silica(8μm)as the matrix and γaminopropyltriethoxysilane as the coupling agent,a stationary phase of biospecific affinity chromatography was prepared by chemical binding nicotinamide adenine dinucleotide(NAD) to the γ-aminopropyl modified silica. Using the phosphate buffer(pH=7.0)as a mobile phase,the separation of some nucleotides is accomplished by the detection of UVD(260 nm).The effect of changed ion strengh of eluent on the resolution of nucleotide(AMP, ADP and ATP)has been studied. |
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| Keywords: | NAD Affinity chromatography Nucleotide |
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