Adsorptive immobilization of intestinal brush border membrane on triton X-100-substituted sepharose 4B |
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Authors: | Mehran Habibi-Rezaei Mohsen Nemat-Gorgani |
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Institution: | (1) Institute of Biochemistry and Biophysics, University of Tehran, PO Box 13145-1384, Tehran, Iran;(2) Present address: Faculty of Science, University of Tehran, Tehran, Iran |
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Abstract: | Triton X-100-substituted Sepharose 4B (Sepharose-TX) was used for adsorptive immobilization of intestinal brush border membrane
using lactose-phlorizin hydrolase as a representative membrane enzyme. Limited heating of membrane preparations was found
to enhance binding. This enhancement is concluded to be owing to a greater availability of the hydrophobic sites, as also
confirmed by the 1-anilino-8-naphthalene sulfonate fluorescence studies, for interaction with Triton X-100 moieties on the
support. The immobilized preparations obtained by this procedure were found useful in hydrolysis of lactose, involving lactose-phlorizin
hydrolase, in continuous operations. It is suggested that the approach may be of general utility for immobilization of biologic
membranes by interaction of their extramembrane structures using supports with appropriate hydrophobic groups. |
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Keywords: | Adsorption continuous operation ectoprotein intestinal brush border membrane lactase-phlorizin hydrolase molten globule |
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