Die mikrophotometrische bestimmung von kobalt im blutserum und anderen biologischen substanzen

A photometric method is proposed for the determination of cobalt in the form the nitroso-R-salt complex in 5–10 nil of blood serum. The organic matter is destroyed by mineral acids. Iron and copper are separated by oxine and dithizone; they can be determined later. Cobalt (just like traces of other elements), complexed by diethyldithiocarbamate and oxine, is extracted by chloroform and then determined in, the form of the nitroso-R-salt complex. The photometric measurement is carried out in a capillary cuvette 10 cm long and 0.7 ml volume, using a green Literatur S. 561. filter. Amounts of 0.02–0.3 g cobalt can be determined quantitatively, with an accuracy of ±5%.