Porous composite scaffolds of hydroxyapatite/silk fibroin via two‐step method |
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Authors: | Lin Liu Jinying Liu Xiangdong Kong Yurong Cai Juming Yao |
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Affiliation: | 1. The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textile, Zhejiang Sci‐Tech University, Hangzhou 310018, China;2. College of Life Sciences, Zhejiang Sci‐Tech University, Xiasha Higher Education Park, Hangzhou 310018, China |
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Abstract: | A two‐step method was used to fabricate the hydroxyapatite (HAP)/silk fibroin (SF) scaffolds, i.e. the nano‐sized HAP/SF composite powders were prepared by co‐precipitation, which were then blended with SF solution to fabricate the HAP/SF composite scaffolds. The obtained scaffolds showed a 3D porous structure. The porosity was higher than 90% with the average macropore size of 214.2 µm. Moreover, the nano‐sized HAP/SF composite powders were uniformly dispersed in the silk fibroin matrix, which provided the scaffolds enhanced compressive properties. The cell culture assay showed that the scaffolds fabricated by the two‐step method could improve the cell proliferation and osteogenic differentiation when compared with those prepared by the conventional one‐step blending method. The results suggested that the two‐step method could promote the uniform dispersion of HAP in the SF matrix and efficient combination between the HAP and the matrix, which may provide a potential application in the composite scaffold preparation for tissue engineering. Copyright © 2009 John Wiley & Sons, Ltd. |
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Keywords: | silk fibroin composite scaffolds two‐step method mechanical properties biocompatibility |
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