NMR研究水溶液中L-精氨酸与磷酸腺苷的相互作用及其构象变化

利用核磁共振(NMR)方法,研究了水溶液中L-精氨酸分别与一磷酸腺苷、三磷酸腺苷的相互作用及其构象变化.通过测定L-精氨酸分别与一磷酸腺苷、三磷酸腺苷混合后体系的1H、13C NMR,对混合前后L-精氨酸1H及13C化学位移变化情况进行了分析,发现L-精氨酸与一磷酸腺苷、三磷酸腺苷存在氢键和静电作用,并且这种相互作用会导致L-精氨酸构象产生变化,即由作用之前的伸展型转变为作用后的弯曲型.

NMR Studies on Conformational Change of L-Arginine Induced by the Interaction with Adenosine phosphate in Aqueous Solution

In this paper, the conformational change of L-Arginine induced by the interaction with Adenosine phosphate in aqueous solution was studied by using NMR technique. The changes of ^1H, ^13C chemical shifts of L-Arglnine due to the presence of Adenosine phosphate were measured. It found that there is interaction of hydrogenbonding and electrostatic attraction between L-Arginine and Adenosine phosphate. Conformational change of L-Ar- ginine molecular have been leaded from this interaction.