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Simple design of an aligned transparent biofilm by magnetic particles and its cellular study |
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| Authors: | Saeed Heidari K Esmaeil Biazar S Meysam Seyedbarzegar Nayerehsadat Mousavi Fina Vosoughi Naghmeh Khademi S Fariba Nami Hesam Hosseinkazemi |
| Institution: | 1. Ophtalmoproteomics Lab, Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran;3. Department of Electric power Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran;4. Department of Biomaterials Engineering, Amirkabir University of Technology, Tehran, Iran |
| Abstract: | The aim of this study was to produce aligned biodegradable films. In this study, we used magnetic microparticles and strong magnetic field for orientation of gelatin gels. The samples were evaluated by microscopic analyses and cell culture assays with Schwann cells. Results of structural analyses showed a good arrangement and orientation of films under strong magnetic field with movement of magnetite particles. Cellular experiments showed a good cell adhesion and orientation on the designed films compared with those on unmodified ones. This aligned guide appears to have the right organization for testing in vivo nerve tissue engineering studies. Copyright © 2016 John Wiley & Sons, Ltd. |
| Keywords: | orientation gelatin magnetic field magnetite particles Schwann cell |