Novel extracellular polymeric substances produced by Cupriavidus necator IPT 027 grown on glucose and crude glycerol originated from biodiesel |
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| Authors: | Paulo Leonardo Lima Ribeiro Márcio Inomata Campos Janice Izabel Druzian |
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| Institution: | 1. Department of Chemical Engineering, Polytechnic School, Federal University of Bahia, Salvador, BA, Brazil;2. Department of Technology, State University of Feira de Santana, Feira de Santana, BA, Brazil;3. Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, BA, Brazil |
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| Abstract: | The aim of this study was to evaluate the production and properties of novel extracellular polymeric substances (EPS) from glucose (EPS 1) and crude glycerol (CG) from castor beans oil (EPS 2). Cupriavidus necator IPT 027 cultivated with glucose and CG (35 °C, 150 rpm, 72 hr, pH 7, shaker) produced approximately 1.60 ± 0.01 and 2.83 ± 0.02 g l?1 EPS, respectively, with high‐molecular weight (3.89 × 105 and 1.89 × 107 Da) and constituted of different functional groups such as uronic acid, monosaccharides (glucose, mannose, arabinose, and fucose) and primary amine group. The composition of the fermentable substrate influenced the melting temperature (221.11 and 230.18 °C), crystallinity (34.36% and 37.11%), degradation temperature (255.06 and 296.62 °C) and morphology. EPS showed pseudoplastic non‐Newtonian fluid behavior in the aqueous solutions, presenting potential applicability biotechnological and industrial mainly in food industry as emulsifiers and biosurfactants. This is the first study on the production and characterization of EPS obtained by C. necator IPT 027 in culture with glucose and CG. Copyright © 2016 John Wiley & Sons, Ltd. |
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| Keywords: | exopolymer thermal properties GC– MS pseudoplastic alternative substrate |
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