The effect of zinc(II) on the growth of <Emphasis Type="Italic">E . coli</Emphasis> studied by microcalorimetry |
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| Authors: | Email author" target="_blank">J?YaoEmail author Y?Liu H?G?Liang C?Zhang J?Z?Zhu X?Qin M?Sun S?S?Qu Z?N?Yu |
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| Institution: | (1) School of Environmental Studies, China University of Geosciences, Wuhan, Hubei, 430074, P. R. China;(2) Department of Chemistry, College of Chemistry and Molecular Science, Wuhan University, Wuhan, Hubei, 430072, P. R. China;(3) Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Huazhong Agricultural University, Wuhan, Hubei, 430070, P. R. China |
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| Abstract: | By using an LKB2277 Bioactivity Monitor, stop-flow mode, the power-time curves of E . coli at 37°C effected by zinc(II) were determined. Some parameters, such as growth rate constants k, inhibitory ratio I, the maximum heat production rate P
max heat output Q and the time in the maximum heat production t
max were obtained. According these parameters, we found that a low concentration of zinc(II) had a promoting action on the growth of E. coli, but a high concentration of zinc(II) had an inhibitory action. The toxicity of zinc(II) can also be expressed as half inhibitory concentration IC
50 of zinc(II), i.e., 50% effective in this inhibition. The value of IC
50 of zinc(II) on E. coliis 28.09 µg mL-1. The assay is quantitative, inexpensive and versatile. |
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| Keywords: | microcalorimetry E coli effect of zinc(II) metabolic action |
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