Study of interaction of a fluorescent probe with DNA |
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| Authors: | Fang-Ying Wu Yan-Ling Xiang Yu-Mei Wu Fei-Yan Xie |
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| Institution: | aDepartment of Chemistry and Center of Analysis and Testing, Nanchang University, Nanchang, 330031, China;bPackaging Engineering Institute of Jinan University, Zhuhai 519070, China |
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| Abstract: | The zinc complex of 2-2-(3, 5-bis(2-pyridylmethyl)aminomethyl-4-hydroxy-phenyl) ethylene]-5-methylpyrazine (1) could bind with the calf thymus deoxyribonucleic acid (ct-DNA). The binding behaviors between them were studied by fluorescence and absorption spectral assay. The absorption titration of 1-Zn with ct-DNA showed no bathochromic shift and hypochromic effect. No anisotropy increase was observed when ct-DNA was added to 1-Zn solution. They both proved the lack of intercalation interaction between 1-Zn and ct-DNA. The ionic strength experiment, Scatchard plot, study of interaction between 1-Zn and denatured ct-DNA all revealed that the interaction mode between 1-Zn and ct-DNA was electrostatic interaction. Binding constant was estimated to be 7.96×104 L moL−1. |
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| Keywords: | 2-[2-(3 5-bis(2-pyridylmethyl)aminomethyl-4-hydroxy-phenyl)ethylene]-5-methyl- pyrazine ct-DNA Fluorescent probe Electrostatic interaction |
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