Development of ERK Activity Sensor,an <Emphasis Type="Italic">in vitro</Emphasis>, FRET-based sensor of Extracellular Regulated Kinase activity |
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Authors: | Harry?M?Green Email author" target="_blank">José?Alberola-IlaEmail author |
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Institution: | (1) Division of Biology, California Institute of Technology, M/C 147-75, 1200 E.California Blvd, Pasadena, CA 91125, USA |
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Abstract: | Background Study of ERK activation has thus far relied on biochemical assays that are limited to the use of phospho-specific antibodies
and radioactivity in vitro, and analysis of whole cell populations in vivo. As with many systems, fluorescence resonance energy transfer (FRET) can be utilized to make highly sensitive detectors of
molecular activity. Here we introduce FRET-based ERK Activity Sensors, which utilize variants of Enhanced Green Fluorescent
Protein fused by an ERK-specific peptide linker to detect ERK2 activity. |
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Keywords: | |
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