Investigation of metabolic changes in blood and tissue of mice γ-irradiated with sublethal doses by direct observation of EPR signals from Hb-NO complexes |
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Authors: | M I Ibragimova V Yu Petukhov E P Zheglov G V Konjukhov R N Nizamov |
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Institution: | (1) Laboratory of Experimental Surgery, Department of General Surgery, Rio de Janeiro State University, Rio de Janeiro, Brazil;(2) Rua Dr. Crespo 10, Cond. Porto Fino 20/casa 01, Recreio, Rio de Janeiro, RJ, 22790-670, Brazil; |
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Abstract: | The metabolic changes in probes of blood and tissue (spleen, liver and kidney) of mice under total γ-irradiation with the
doses varied in the interval of 1–10 Gy at the dose rate of 0.073 Gy/min were studied in the early postirradiation period
by ex vivo electron paramagnetic resonance (EPR). It was established that the impact with the lower dose rate leads to more
intensive nitric monoxide biosynthesis in comparison with higher dose rates. In the early postirradiation period (from 2 up
to 6 h), irradiation with doses higher than 2 Gy brings about an increase of the NO concentration and, hence, the appearance
of nitrosyl complexes which were registered directly by EPR in blood and spleen. The observed line is identified as the signal
from α-(Fe2+-NO)2β(Fe3+)2 or α-(Fe2+-NO) α(Fe2+)β(Fe3+)2 complexes since the methemoglobin concentration also increases in comparison with the control level. The concentration of
Hb-NO complexes in blood and spleen depends on the dose and individual radiosensitivity of the organism. Therefore, the intensity
of the Hb-NO signal may serve as a criterion of the radiation injury level during the first hours after the irradiation. 30
h after the impact, the Hb-NO complexes were no longer detected. For the first day, the concentration of Fe3+-transferrin in blood increases with the dose and time passed after the irradiation. The intensity of the EPR signal from
Fe3+-transferrin in blood may also serve as a measure of the radiation injury level. |
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