Gas-phase scrambling of disulfide bonds during matrix-assisted laser desorption/ionization mass spectrometry analysis |
| |
| Authors: | Liang Zhao Ruben T Almaraz Fan Xiang Jerry L Hedrick Andreas H Franz |
| |
| Institution: | aDepartment of Chemistry, University of the Pacific, Stockton, California, USA;bDepartment of Animal Science, University of California Davis, Davis, California, USA;cShimadzu Biotech Corporation, Pleasanton, California, USA |
| |
| Abstract: | Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization
mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin
and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K
was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was
found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of
disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous
assignment of disulfide bonds. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect SpringerLink 等数据库收录！ |
|
