Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide‐Based Chemosensing Ensemble |
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Authors: | Dr Qingtao Meng Hongmin Jia Xue Gao Yue Wang Dr Run Zhang Dr Renjie Wang Prof Zhiqiang Zhang |
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Institution: | 1. Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, P.R. China;2. Department of Chemistry and Biomolecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney NSW, Australia;3. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia |
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Abstract: | We described a new ensemble‐approach‐based chemosensor, NCH‐Cu2+, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu2+ ions over other metal ions, accompanied with a 92.2 % fluorescence quenching. The decomplexation of NCH‐Cu2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH‐Cu2+ towards His showed a good linearity with a detection of limit at 70 nm . Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH‐Cu2+ towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH‐Cu2+ was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level. |
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Keywords: | chemosensor fluorescence imaging histidine lysosome quantification |
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