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糯米藤的离体快繁系统的建立
引用本文:李珉,曾涛,张义正. 糯米藤的离体快繁系统的建立[J]. 四川大学学报(自然科学版), 2002, 39(2): 356-360
作者姓名:李珉  曾涛  张义正
作者单位:四川大学生命科学学院,成都610064
摘    要:对糯米藤(Memorialis hirta BL.Wedd.)进行了离体快繁研究。叶片和茎段在附加NAA0.5mg/L+KT1.0mg/L或NAA0.5mg/L 6-BA 1.0mg/L的MS培养基上,诱导形成愈伤组织后再分化形成植株;腋芽在附加NAA0.1mg/L KT1.0mg/L或NAA0.1mg/L 6-BA1.0mg/L或IAA0.1mg/L 6-BA 1.0mg/L的MS培养基上诱导出丛生芽,并再生成植株。

关 键 词:糯米藤 组织培养 离体快繁 中药 愈伤组织 从生芽 再生植株
文章编号:0490-6756(2002)02-0356-05

The construction of quick reproduction system in vitro of Memorial hirta Bl. Wedd
LI Min,ZENG Tao,ZHANG Yi-zheng. The construction of quick reproduction system in vitro of Memorial hirta Bl. Wedd[J]. Journal of Sichuan University (Natural Science Edition), 2002, 39(2): 356-360
Authors:LI Min  ZENG Tao  ZHANG Yi-zheng
Abstract:The quick reproduction system in vitro of Memorialis hirta BL. Wedd was studied. Leaves and stems were induced into calli and then differentiated into regeneration plants on MS media supplemented with NAA 0.5mg/L+KT 0.1mg/L or NAA 0.5mg/L +6-BA 1.0mg/L. Axillary buds induced into bud clusters and then differentiated into regeneration plants on MS media supplemented with NAA 0.1mg/L + KT 1.0mg/L or NAA 0.1mg/L+6-BA 1.0mg/L or IAA 0.1mg/L+6-BA 1.0mg/L.
Keywords:Memorial hirta(BL.) Wedd.  tissue culture  quick reproduction in vitro
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