水杨酸对烟草GRPs基因表达的影响

通过RT-PCR得到富含甘氨酸RNA结合蛋白（GRPs）基因片段，利用northern杂交技术对水杨酸诱导不同时间不同烟草GRPs基因的表达模式进行研究.结果表明：水杨酸能诱导烟草GRPs基因上调表达，3 h达到最高，然后开始下降；但3个TMV抗性不同的供试烟草增加幅度不同，基因表达幅度与抗性有一定关系,抗性强的红花大金元表达增幅大于抗性较弱的云烟85.本研究明确了水杨酸诱导下烟草该基因的表达谱，揭示了SA诱导烟草不同抗性的品种的幅度不同，并且发现诱导后3 h为关键点，表明GRPs基因可作为烟草水杨酸代谢途径中的一个标记基因研究其途径对逆境的应答.

The Effect of Salicylic Acid on Expression of the Gene Coding Glycine-Rich Protein Binding RNA in Tobacco

The gene fragments(GRPs) coding glycine-rich protein binding RNA in tobacco materials were got by RT-PCR.Northern hybridization blot analysis was used to demonstrate the expression pattern of the GRP gene at different times after treatment of salicylic acid in different tobacco materials.Inducded by salicylic acid,the GRP genes of Nicotiana could up-regulate expression.The expression attained the highest in 3h,then began descending.The three tobacco varieties had different resistance abilities to TMV.The expression level of two genes correlated with the resistance ability.The increase of two genes in Honghuadajingyuan with stronger resistance ability was higher than that of Yunyan 85 with less resistance ability.The expression pattern of the gene induced by salicylic acid in tobacco was got.It was found that in different tobacco varieties with different resistance abilities,the up-regulation extents of expression of the GRP gene were quite different after treatment of salicylic acid.And it is also found that the up expression became significant after 3 hours treatment.From the results of this study,it is suggested that GRP gene can be used as a marker gene to investigate salicylic acid metabolism in tobacco varieties so as to demonstrate their response to abiotic stresses.