| 外源柠檬酸缓解大豆根系短期铝胁迫的FTIR特征分析 |
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| 引用本文： | 金婷婷,刘鹏,张志祥,徐根娣,赵莉莉.外源柠檬酸缓解大豆根系短期铝胁迫的FTIR特征分析[J].光谱学与光谱分析,2009,29(2):367-371. |
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| 作者姓名： | 金婷婷 刘鹏 张志祥 徐根娣 赵莉莉 |
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| 作者单位： | 浙江师范大学植物学实验室, 浙江 金华 321004 |
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| 基金项目： | 国家科技攻关项目，国家自然科学基金，浙江省自然科学基金 |
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| 摘 要： | 选择耐铝性不同的2个大豆品种浙春2号(耐铝性)和浙春3号(铝敏感)作为实验材料，在设置铝胁迫的同时添加外源柠檬酸处理，应用傅里叶变换红外光谱法(FTIR)对外源柠檬酸协同铝胁迫下的大豆根组织干燥粉末进行直接测定，并对其特征吸收峰进行了归属。通过对各图谱的比较分析，铝处理下大豆根的红外吸收峰形及峰强有所区别，特别是1 057, 1 602, 2 927和3 297 cm-1左右的吸收峰区别明显，反应出蛋白质，糖类以及核酸等有机物的含量变化；以A2 927 cm-1/A3 297 cm-1为横坐标、A1 051 cm-1/A1 602 cm-1为纵坐标而绘制的二维分析图显示，外源柠檬酸的加入使不同处理的红外吸收峰形及峰强差异降低，意味着有机物等有效成分的变化不显著，且浙春3号尤为明显。实验表明FTIR在某种程度上能够反映外源柠檬酸对铝胁迫下大豆根的缓解效应，为该领域的研究工作提供了有益的参考。
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| 关 键 词： | FTIR 直接测定 外源柠檬酸 铝胁迫 大豆根 |
| 收稿时间： | 2007-09-28 |
Analysis of Roots of Soybean (Glycine max Merrill) Treated with Exogenous Citric Acid Plus Short-Time Aluminum Stress by Direct Determination of FTIR Spectrum |
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| JIN Ting-ting,LIU Peng,ZHANG Zhi-xiang,XU Gen-di,ZHAO Li-li.Analysis of Roots of Soybean (Glycine max Merrill) Treated with Exogenous Citric Acid Plus Short-Time Aluminum Stress by Direct Determination of FTIR Spectrum[J].Spectroscopy and Spectral Analysis,2009,29(2):367-371. |
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| Authors: | JIN Ting-ting LIU Peng ZHANG Zhi-xiang XU Gen-di ZHAO Li-li |
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| Institution: | Key Laboratory of Botany, Zhejiang Normal University, Jinhua 321004, China |
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| Abstract: | In the present study,19 soybean(Glycine max L.) cultivars were analyzed and found to differ considerably in aluminum(Al) resistance.The cultivars Zhechun No.2(Al-resistant) and Zhechun No.3(Al-sensitive) were selected for further analysis.Experiments were performed with plants grown in full nutrient solution for 30 days.Fourier transform infrared spectrometry(FTIR) with OMNI-sampler was applied to the direct determination of different varieties of soybean root tissues,treated with aluminum in a dose-and tim... |
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| Keywords: | FTIR |
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