Impedimetric genosensors for the detection of DNA hybridization |
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| Authors: | A. Bonanni M. J. Esplandiu M. I. Pividori S. Alegret M. del Valle |
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| Affiliation: | (1) Sensors and Biosensors Group, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Bellaterra, Barcelona, Spain |
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| Abstract: | Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands. In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical reaction serves as the working signal, allowing for an unlabelled gene assay.  |
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| Keywords: | DNA biosensor Impedance Capacitance Unlabelled DNA |
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