整体式PDMS电泳芯片快速成型及高灵敏化学发光检测氨基酸

通过再铸模法将聚二甲基硅氧烷(PDMS)预聚物固化在由微细金属丝构成的微流体孔道的印模中,一次成型制作了整体式PDMS芯片.将所制作的芯片与化学发光检测器集成构建了微芯片毛细管电泳分析系统.初步考察了不经过衍生化时该系统分离检测氨基酸的性能.实验结果表明,精氨酸和天门冬氨酸在80s内完全分离,分离度为2.45,精氨酸的浓度检测限为3.50μmol/L.

Rapid Fabrication of Monolithic PDMS Microchips and Highly Sensitive Chemiluminescence Detection for Amino Acids

A novel and facile method for the fabrication of monolithic microfluidic chips in poly dimethylsiloxane)(PDMS) has been presented. The fabrication consists of two steps including channels definition and replica molding. Two micrometeric metal wires, previously welded in the cross section on a co-plane, were spanned over a templet to define microscopic channels. PDMS is then cast against the templet. After the metal wires removed and the templet peeled away, a monolithic replica with enclosed networks of channels is obtained. Scanning electron microscopy and CCD imaging demonstrated that the cross-section of two channels was complete contact and channel inner well is smooth. A microchip capillary electrophoresis system was constructed by integrating the monolithic microchip with a chemiluminescence detection, and its performances was demonstrated by the separation and the detection of two amino acids such as arginine and aspartic acid without derivatization procedures. Excellent separation with the resolution of 2.45 within 80 s was achieved with the concentration detection limit(S/N=3) of 3.50 μmol/L for arginine.