电化学发光猝灭法测定谷胱甘肽

谷胱甘肽是生物体内的重要活性物质，它的研究测定对人类的健康和生活具有重要的意义。文章在中性介质中鲁米诺-碘化钾电化学发光的基础上，研究了还原型谷胱甘肽对此电化学发光反应的猝灭作用，并在此基础上建立了一种高灵敏度的谷胱甘肽的新的测定方法。该方法测定谷胱甘肽的线性范围为3.38×10-13～4.72×10-3 mol·L-1, 是迄今为止见诸报道的对谷胱甘肽测定的最灵敏的方法，也是测定范围最宽的方法。

The Determination of Glutathione by Electrochemiluminescence Quenching Method

Glutathione is an important active substance in organisms. Its study and determination are very significant to human being's health and life. In neutral medium, the quenching effect of reduced glutathione on the electrochemiluminescence of luminol-iodide was studied in this paper. Therefore a novel sensitive method for the determination of reduced glutathione was developed based on it. The linear response range of this method is from 3.38 x 10(-13) mol x L(-1) to 4.72 x 10(-3) mol x L(-1). This method is the most sensitive one and its linear range is the widest among those reported in the literature.