Separate quantification of doubly and singly 13C-labeled metabolites by HSQC-filtered J spectroscopy |
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Authors: | Davison A P Jones J A Dixon R M |
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Institution: | Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom. |
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Abstract: | NMR detection of multiply labeled compounds in biological samples is often used to follow metabolic pathways. Detection of protons bound to 13C atoms offers a more sensitive approach than direct 13C detection, but generally results in the loss of carbon-carbon coupling information. We have modified an HSQC sequence to refocus the carbon chemical shifts in order to obtain a proton-correlated 13C homonuclear J spectrum, which allows us to measure singly and doubly labeled compounds in the same spectrum. |
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