| 流动注射分光光度法快速测定乳制品中的蛋白质含量 |
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| 引用本文： | 梁琴琴,李永生. 流动注射分光光度法快速测定乳制品中的蛋白质含量[J]. 分析化学, 2011, 0(9): 1412-1417 |
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| 作者姓名： | 梁琴琴 李永生 |
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| 作者单位： | 四川大学化学工程学院; |
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| 摘 要： | 建立了快速测定乳制品中真蛋白质含量的交替合并带异步注入流动注射分光光度法.样品和试剂自动同时定量,分别同时被注入到超纯水和缓冲液中,以合并带中试样包裹试剂的方式进行汇合、反应,生成的蓝色络合物进入流通检测器,在595 nm下进行定量分析.得到的最佳测定条件为:反应试剂中考马斯亮蓝的浓度为150 mg/L,缓冲液中HCI...
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| 关 键 词： | 流动注射分析 合并带异步注入 分光光度法 考马斯亮蓝 蛋白质测定 乳制品 |
Rapid Determination of Real Protein Content in Dairy Products by Flow Injection-Spectrophotometry |
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| LIANG Qin-Qin,LI Yong-Sheng. Rapid Determination of Real Protein Content in Dairy Products by Flow Injection-Spectrophotometry[J]. Chinese Journal of Analytical Chemistry, 2011, 0(9): 1412-1417 |
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| Authors: | LIANG Qin-Qin LI Yong-Sheng |
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| Affiliation: | LIANG Qin-Qin,LI Yong-Sheng (School of Chemical Engineering,Sichuan University,Chengdu 610065) |
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| Abstract: | Based on asynchronous-injection alternating merging zone technology,a flow-injection spetrophotometry for fast determination of real protein content in dairy products was established.Sample and coomassie brilliant blue G250(CBBG) solution were loaded into loops simultaneously and automatically,injected into two carriers,respectively,then merged and reacted in manner of sample enwrapping reagent,and the formed blue complex was determined at 595 nm.Optimized conditions were as follows: concentrations of CBBG,... |
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| Keywords: | Flow injection analysis Asynchronous-injection merging zone Speotrophotometry Coomassie brilliant blue Protein determination Dairy products |
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