| 鱼鳔膜为基质的生物传感器测定葡萄糖的研究 |
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| 引用本文： | 贾文娟,崔淼,张彦,双少敏. 鱼鳔膜为基质的生物传感器测定葡萄糖的研究[J]. 分析化学, 2011, 0(9): 1423-1426 |
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| 作者姓名： | 贾文娟 崔淼 张彦 双少敏 |
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| 作者单位： | 山西大学化学化工学院; |
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| 基金项目： | 国家自然科学基金(No.90813018); 山西省回国留学基金资助 |
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| 摘 要： | 以鱼鳔膜为基质同定葡萄糖氧化酶,偶联氧电极,构建了葡萄糖生物传感器,通过测定溶解氧浓度的变化定量测定葡萄糖.考察了酶浓度、pH值、缓冲液浓度对传感器的影响,优化了实验条件:即酶浓度为1 mg,pH 7.0,缓冲液浓度为100 mmol/L.此传感器具有较宽的线性范围(0.016～1.2 mmol/L),较短的响应时间(...
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| 关 键 词： | 葡萄糖传感器 鱼鳔膜 葡萄糖氧化酶 |
A Biosensor Based on Swim Bladder Membrane as Substrate for Determination of Glucose |
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| JIA Wen-Juan,CUI Miao,ZHANG Yan,SHUANG Shao-Min. A Biosensor Based on Swim Bladder Membrane as Substrate for Determination of Glucose[J]. Chinese Journal of Analytical Chemistry, 2011, 0(9): 1423-1426 |
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| Authors: | JIA Wen-Juan CUI Miao ZHANG Yan SHUANG Shao-Min |
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| Affiliation: | JIA Wen-Juan,CUI Miao,ZHANG Yan,SHUANG Shao-Min (School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006) |
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| Abstract: | A glucose biosensor based on the swim bladder immobilized glucose oxidase has been deve-loped.The glucose concentration was quantified by the decrease of dissolved oxygen.The effects of enzyme loading,pH,buffer concentration and interference were studied.When the enzyme concentration was 2.0%,pH was 7.0,and the buffer concentration was 100 mmol/L,the biosensor showed a maximum response.The biosensor showed a wide linear response with a concentration range of 0.016-1.2 mmol/L,fast response time(70 s),good re... |
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| Keywords: | Glucose biosensor Swim bladder Glucose oxidase |
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