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High performance liquid chromatography with immobilized Ru(bpy)_3~(2+)-KMnO_4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes |
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| Authors: | Ai Hua Fu Zhu Jun Zhang Li Li Chen Xiao Ming Zhang Pan Xue Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province School of Chemistry Materials Science Shaanxi Normal University Xi'an China |
| Institution: | Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China |
| Abstract: | A novel high performance liquid chromatography-chemiluminescence(HPLC-CL) method for investigation of in vitro metabolism of repaglinide in pig liver microsomes with microdialysis sampling technique was developed.The chromatographic separation was performed on a Hypersil BDS-C18 column with an isocratic mobile phase consisting of methanol and 0.01 mol/L KH_2PO_4(pH 3.0)(volume ratio 75:25) at a flow rate of 1.0 mL/min.The detection was based on the chemiluminescence reaction of repaglinide with acidic potas... |
| Keywords: | High performance liquid chromatography Chemiluminescence Microdialysis Repaglinide Immobilized Ru(bpy)_3~(2 ) |
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