| 微流控芯片上大肠杆菌的电化学阻抗检测方法研究 |
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| 引用本文： | 彭金兰,徐溢,吴永杰,传娜,甘俊,田鹏.微流控芯片上大肠杆菌的电化学阻抗检测方法研究[J].分析化学,2011(9):1307-1312. |
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| 作者姓名： | 彭金兰 徐溢 吴永杰 传娜 甘俊 田鹏 |
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| 作者单位： | 重庆大学化学化工学院;新型微纳器件与系统技术国防重点学科实验室;微纳系统及新材料技术国际研发中心;重庆大学; |
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| 基金项目： | 全国博士学位论文作者专项资金基金(No.200941); 国家科技部国家科技部项目(No.2010ZX09401-306-1-5); 中央高校基本科研业务费(No.CDJXS1-22-00-05); 重庆大学研究生科技创新基金项目(No.CDJXS10-22-11-39)资助 |
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| 摘 要： | 基于细菌悬浮液的阻抗特性和微流控芯片电化学阻抗检测技术,采用所构建的微流控芯片电化学阻抗分析系统,以大肠杆菌为样本,优化了扰动振幅、缓冲溶液电导率、扫描频率和新制大肠杆菌标本静置时间等参数,对大肠杆菌标本、大肠杆菌标准合成样本进行电化学阻抗检测,建立了一种大肠杆菌快速定量检测方法.在扰动振幅500 mV、缓冲溶液电导率...
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| 关 键 词： | 大肠杆菌 微流控芯片 微流控电化学阻抗分析系统 定量检测 |
Determination of E.coli with Electrochemical Impedance on Homemade Microfluidic Chip |
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| PENG Jin-Lan,XU Yi,WU Yong-Jie,CHUAN Na,GAN Jun,TIAN Peng.Determination of E.coli with Electrochemical Impedance on Homemade Microfluidic Chip[J].Chinese Journal of Analytical Chemistry,2011(9):1307-1312. |
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| Authors: | PENG Jin-Lan XU Yi WU Yong-Jie CHUAN Na GAN Jun TIAN Peng |
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| Institution: | PENG Jin-Lan1,2,XU Yi1,3,WU Yong-Jie1,CHUAN Na1,GAN Jun1,TIAN Peng2,3 (Chemistry and Chemical Engineering College1,Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology2,International R & D center of Micro-nano Systems and New Materials Technology3,Chongqing University,Chongqing 400030) |
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| Abstract: | Based on the impedance properties of bacterial suspension and microfluidic impedance analytical technology on microchip,the homemade microfluidic impedance analytical system and a rapid and simple quantitative analytical method of bacterial were proposed.In the experiment,E.coli was taken as the detecting sample.Some parameters,such as amplitude of perturbation,conductivity of buffer,scanning frequency range and time of deposition,were optimized.The impedance signals of the standards and the composite sampl... |
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| Keywords: | E coli Microfluidic chip Homemade microfluidic impedance analytical system Quantitative detection |
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