| 小鼠液胞H~+-ATPase 15 K启动子(M15 K)的克隆及其在植物体内的表达特性研究 |
| |
| 引用本文： | 岳文冉,于秀敏,王瑞刚.小鼠液胞H~+-ATPase 15 K启动子(M15 K)的克隆及其在植物体内的表达特性研究[J].河南师范大学学报(自然科学版),2014(2):119-123. |
| |
| 作者姓名： | 岳文冉 于秀敏 王瑞刚 |
| |
| 作者单位： | 内蒙古农业大学生命科学学院; |
| |
| 基金项目： | 内蒙古自然科学基金重点项目(2010Zd13);国家自然科学基金项目(31060105) |
| |
| 摘 要： | PCR克隆了小鼠液胞H+-ATPase 15K启动子,构建具有Kan抗性和GUS intron报告基因的植物表达载体LpPMG.通过M15K启动子指导的GUS intron基因在烟草叶片内的瞬时性表达,比较了其植物表达特性.结果表明:M15K启动子可启动GUS在植物体内的表达.其表达活性相当于2×35S启动子的87.0%±17.3%.
|
| 关 键 词： | 植物 M15 K启动子 GUS 瞬时表达 增强型35S启动子 |
Cloning of Mice Vacuole H~+-ATPase 15 K Promoter(M15 K)and the Research of Expression Characteristics in Plants |
| |
| Institution: | ,College of Life Sciences,Inner Mongolia Agricultural University |
| |
| Abstract: | Mice Vacuole H+-ATPase 15K Promoter was amplified by PCR.The recombinant expression plasmid LpPMG which contain Kanamycin resistance and a GUS intron reporter gene was constructed and then transformed into tobacco leaves.Comparing its plant expressional activity by the M15K promoter guided GUS intron transient expression in the tobacco leaves.The results showed that M15K promoter can drive GUS expression in plants.Its expression activity is equivalent to87.0%±17.3% of the enhanced 35S promoter. |
| |
| Keywords: | plant M15K promoter GUS transient expression enhanced 35S promoter |
| 本文献已被 CNKI 等数据库收录！ |
