| Superresolution imaging of DNA tetrahedral nanostructures in cells by STED method with continuous wave lasers |
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| 作者姓名： | Jiancong Du Suhui Deng Shangguo Hou Lingling Qiao Jianfang Chen Qing Huang Chunhai Fan Ya Cheng Yun Zhao |
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| 基金项目： | supported by the National Natural Science Foundation of China under Grand Nos.61008056,21227804,61078016,and 61378062) |
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| 摘 要： | DNA tetrahedral nanostructures are considered to be uew nanocarriers because they can be precisely controlled and hold excellent penetration ability to the cellular membrane. Although the DNA tetrahedral nanostructure is extensively studied in biology and medicine, its behavior in the cells with nanoscale resolution is not understood clearly. In this letter, we demonstrate superrcsolution fluorescence imaging of the distribution of DNA tetrahedral nanostructures in the cell with a simulated emission depletion （STED） microscope, which is built based on a conventional eonfocal microscope and can t）rovide a resolution of 70 nm.
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| 关 键 词： | 纳米结构 受激发射 四面体 DNA 细胞膜 连续波激光器 超分辨成像 损耗 |
| 收稿时间： | 2013/12/17 |
Superresolution imaging of DNA tetrahedral nanostructures in cells by STED method with continuous wave lasers |
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| Jiancong Du,Suhui Deng,Shangguo Hou,Lingling Qiao,Jianfang Chen,Qing Huang,Chunhai Fan,Ya Cheng,Yun Zhao.Superresolution imaging of DNA tetrahedral nanostructures in cells by STED method with continuous wave lasers[J].中国光学快报(英文版),2014,12(4):41101-38. |
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| Authors: | Jiancong Du Suhui Deng Shangguo Hou Lingling Qiao Jianfang Chen Qing Huang Chunhai Fan Ya Cheng and Yun Zhao |
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| Institution: | [1]College of Life Sciences, SiChuan University, Chengdu 610064, China [2]Division of Physical Biology, and Bioimaging Center, Shanghai Synchrotron Radiat ion Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China [3]Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences Shanghai 201800, China |
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| Abstract: | DNA tetrahedral nanostructures are considered to be new nanocarriers because they can be precisely controlled and hold excellent penetration ability to the cellular membrane.Although the DNA tetrahedral nanostructure is extensively studied in biology and medicine,its behavior in the cells with nanoscale resolution is not understood clearly.In this letter,we demonstrate superresolution fluorescence imaging of the distribution of DNA tetrahedral nanostructures in the cell with a simulated emission depletion(STED)microscope,which is built based on a conventional confocal microscope and can provide a resolution of 70nm. |
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| Keywords: | 110 0180 100 6640 180 2520 |
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