Triton X-100微乳液中铈与L-色氨酸荧光反应的研究与应用

研究了Triton X-100微乳液(Triton X-100-M)对荧光法测定L-色氨酸的增敏作用。在酸性条件中Ce4+可将色氨酸氧化为强荧光性物质，以此为基础建立了分析色氨酸的新方法。与Triton X-100胶束相比，Triton X-100微乳液对荧光法测定L-色氨酸具有更好的增敏作用，在该介质中测定灵敏度提高了225倍。实验中对影响荧光测定的各种因素进行了优化，同时考察了共存离子的干扰并做了回收试验，对荧光增敏机理也进行了初步探讨。试验结果表明：在优化条件下，色氨酸浓度在0.1～1.0 μg·mL-1范围内与荧光强度成线形关系，其线性方程为F=342.37+30.45c，r=0.998 4，检出限为0.09 μg·mL-1。在优化参数条件下，对实际样品进行分析，结果令人满意。

Investigation and Application of Fluorescence Reaction of Cerium-L-Tryptophan in Triton X-100 Microemulsion

In the present article, the sensitizing effect of Triton X-100 microemulsion media (Triton X-100-M) on the determination of L-tryptophan by fluorescence spectrophotometry was studied, which was based on a strong fluorescence substance produced when L-tryptophan was oxidized by Ce4+ in acid medium. Compared with Triton X-100 micelle (Triton X-100-m), it was found that in the medium of Triton X-100-M the intensity of fluorescence was enhanced by 225 times. Various factors that influence fluorescence determination and interfering ions were examined. The mechanism of sensitizing effect of Triton X-100 microemulsion was also discussed. Under the optimum conditions, the liner range and detection limit are 0.1-1.0 g x mL(-1) and 0.09 microg x mL(-1) (correlation coefficient r = 0.998 4), respectively. The linear equation was F = 342.37+30.45c. The proposed method has been successfully applied to determine food samples.