云南盘鮈酶水解条件研究

采用动物蛋白水解酶对云南盘鮈进行酶水解,通过正交实验法确定了酶水解条件。结果表明,酶水解的最适条件为:温度50℃,pH7.0,时间3h,加酶量0.5%,固液比1∶2.5。在该条件下盘鮈肉蛋白的水解度为32.85%。氨基酸分析表明,游离氨基酸含量36.47mg/mL,必需氨基酸14.42mg/mL,占39.54%。尤其是亮氨酸、缬氨酸、苏氨酸、赖氨酸含量丰富,为低值鱼深加工研究提供理论依据。

Investigation on the Technological Condition of Controlled Enzymatic Hydrolysis for Discogobio, Yunnanensis

The peptides with lower molecular weight from Discogobio,Yunnanensis（Stone Head Fish）were studied.Through orthogonal design test,the technological conditions of enzymatic hydrolysis of Discogobio,Yunnanensis with enzyme of animal protein hydrolysis were obtained as：hydrolysed temperature 50℃,hydrolysed time 3h,pH 7 and dosage of animal protein hydrolysis enzyme 0.5% based the amount of fish protein,ratio of substrate to water 1∶2.5,the degree of hydrolysis（DH）of product up to 32.85%.The result of amino-acid analysis demonstrated that free amino-acids in product are 36.47mg/mL,essential amino-acids 14.42mg/mL with 39.54% against total amino-acids.Of which,leucine（Leu）,threonine（Thr）and lysine（Lys）are abundant amino-acids in product.The results provide significant data for exploring the resource of Discogobio,Yunnanensis in Yunnan province for health food.