Liquid chromatographic separation of fluoroquinolone antibacterials used as veterinary drugs |
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Authors: | J. A. Hernández-Arteseros I. Boronat R. Compañó M. D. Prat |
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Affiliation: | (1) Departament de Química Analítica, Universitat de Barcelona, Avda Diagonal 647, 08028 Barcelona, Spain |
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Abstract: | Summary A liquid chromatographic method has been developed to separate a series of quinolones which are widely used as veterinary drugs: danofloxacin, difloxacin, enrofloxacin (and its metabolite ciprofloxacin), marbofloxacin, norfloxacin and sarafloxacin. Separation was performed by using an end-capped high-purity silica-based C8 column and a mobile phase consisting of acetonitrile-aqueous oxalic acid buffer solution. In order to establish the optimal conditions for an isocratic separation, a three-factor Doehlert experimental design was applied. Gradient elution was applied which reduced analysis time. Figures of merit of the method proposed, with fluorimetric detection, were evaluated. |
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Keywords: | Column liquid chromatography Doehlert design Dluoroquinolones Residue analysis |
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