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Generating Encoded Compound Libraries for Fabricating Microarrays as a High-Throughput Protein Ligand Discovery Platform
Authors:Yung-Shin Sun  Yiyan Fei  Juntao Luo  Seth Dixon  James P Landry  Kit S Lam
Institution:1. Department of Physics , Fu-Jen Catholic University , New Taipei City , Taiwan;2. Department of Optical Science and Engineering , Fudan University , Shanghai , China;3. Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Ultra-Precision Optical Manufacturing Engineering Center , Fudan University , Shanghai , China;4. Department of Pharmacology , Upstate Cancer Research Institute, State University of New York Upstate Medical University , Syracuse , New York , USA;5. Division of Hematology and Oncology, Department of Internal Medicine , School of Medicine, University of California at Davis , Sacramento , California , USA;6. Department of Physics , University of California at Davis , Davis , California , USA;7. Division of Hematology and Oncology, Department of Internal Medicine , School of Medicine, University of California at Davis , Sacramento , California , USA;8. Department of Biochemistry and Molecular Medicine , School of Medicine, University of California at Davis , Sacramento , California , USA
Abstract:We demonstrate an effective method for generating libraries of encoded compounds for fabricating large compound microarrays on solid supports. This method is based on one-bead, one-compound synthesis and employs a novel trilayer bead-partition scheme that ensures sufficient quantity of synthesized compounds releasable from each bead for compound microarray fabrication in high-throughput protein–ligand discovery assays.

Supplementary materials are available for this article. Go to the publisher's online edition of Synthetic Communications® for the following free supplemental resource(s): Full experimental and spectral details.]

Keywords:Chemical microarray  high-throughput screening  one-bead–one-compound (OBOC) synthesis  protein–ligand binding assay
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