超微弱发光用于番茄果实冷害发生程度的无损监测

超微弱发光测定法可快速、灵敏地反映生物体在逆境下的生理状态,已开始得到广泛应用,但有关其在采后园艺产品冷害发生监测中的应用尚未见报道.文章通过分析番茄果实在不同冷害状态下的超微弱发光规律,及其与冷害程度问的关系,探讨超微弱发光测定法在监测采后番茄果实冷害中应用的可能性.结果表明,果实超微弱发光强度随冷害程度的提高而上升,且在表现冷害症状前,冷害果实的超微弱发光值已显著高于正常果实(P<0.01).不同冷害程度下果实的冷害发病率、冷害指数分别与超微弱发光值的相关系数达到0.901 6和0.957 7(P<0.01),证明超微弱发光可以准确反映番茄果实冷害程度.

Nondestructive examination of tomato chilling injury by ultraweak luminescence

Ultraweak luminescence (UWL) is an universal phenomenon of photons emission in living organism. It is intrinsically connected with biological processes, and it closely links with biochemical, physiological and pathological conditions in organisms under stress. UWL testing is widely used in clinical medicine, agriculture, environmental protection and food industry etc. But it was not applied in testing physiological disease of agricultural product in storage. This study used tomato fruit as material to find rule of UWL in fruits under chilling stress, which is the main problem in tomato fruit storage. To obtain fruits with different chilling injury degree, before cold storage a group of fruits were treated with cold shock, the effective method to decrease chilling injury, which showed alleviative chilling injury symptom compared with untreated fruits. It was found that UWL rose with the degree of chilling injury, furthermore UWL went up remarkably (P < 0.01) before symptom of chilling injury happening. Correlation analysis between UWL and chilling injury rate and chilling injury index showed that correlation coefficient was 0. 901 6 and 0.9577 (P < 0.01)respectively. In conclusion, UWL could factually reflect the degree of chilling injury.