lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies |
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Authors: | E. Grell E. Lewitzki C. Bremer S. Kramer-Schmitt J. Weber A. E. Senior |
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Affiliation: | (1) Max-Planck-Institute of Biophysics, Kennedyallee 70, 60596 Frankfurt, Germany;(2) Department of Biochemistry, University of Rochester Medical Center, 14642 Rochester, New York |
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Abstract: | lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties oflin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The pK values of the Mg2+ and Ca2+ complex oflin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.This is a peer-reviewed conference proceeding article from the Third Conference on Methods and Applications of Fluorescence Spectroscopy, Prague, Czech Republic, October 18–21, 1993. |
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Keywords: | lin-Benzo-ATP lin-benzo-ADP dissociation constants Mg2+ and Ca2+ binding fluorescence spectra |
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