应用不同光谱技术,动物RBC,Hb及人皮肤表面血流氧化-还原状态检测

目的:检测与分析实验动物血液RBC(RBC·O2,RBC·CO2),Hb(HbO2,HbCO2)和人体皮肤表面流动血液氧化·还原状态的成像与未成像可见光谱领域OD值特征,并为该技术对白癜风病表皮黑色素颗粒检测中的应用尊定基础。方法:利用不同光谱技术和invitro和invivo检测手段,统计分析血液不同状态下波长与位置的OD值信息。结果:invitro检测:动物血液Hb·O2和RBC·O2两者在可见领域均有367,414(Soret带)nm与541,576(Q带)nm的吸收峰位;血液Hb·CO2和RBC·CO2均有432(Soret带)与和553(Q带)nm的波长吸收峰位;血液RBC状态和Hb溶血状态波长吸收峰位无改变,只是在氧化与还原状态下有完全独立的吸收峰位,血液RBC状态和Hb溶血状态波长吸光度OD值之间,有显著性差异(p<0·01)。浓度为1·5×107cell·mL-1的RBC·O2和Hb·O2在576nm的吸收峰位吸光度(y)与红细胞浓度(x)做成两条回归曲线:既,Hb·O2(b1)^y=0·05 0·983x;RBC·O2(b2)y^=0·127 1·934x,两者之间差异有显著性(p<0·01)。invivo检测:在人手背皮肤表面ImSpector图像中RBC·O2状态在540,576nm,RBC·CO2状态在555和755nm处有吸收峰。选择(a:指甲,b:指,c:手背)三个点位分别进行波长检测,每点(n=10)545nm吸收峰的平均OD值,依次为0·83±0·001,0·73±0·001和0·62±0·001,其三处测定点的OD值之间有显著性差异(p<0·01)。结论:invitro检测的RBC与Hb两者波长吸收峰位不变,但吸光度OD值不同,认为RBC状态测定结果更接近于活体组织血管内原始状态。invivo检测对人体无任何侵袭与损伤,灵敏度高,测试时间短,并且同时获得被测样品的波长与位置信息画面等优势,有望表皮中黑素等有色颗粒的直接检测。

Examination of Animal RBC,Hb and Human Skin Surface Blood Stream in Oxygenation-Deoxidization Conditions Using Different Spectrum Techniques

The authors examined and analyzed animal blood RBC (RBC. O2, RBC. CO2), Hb (HbO2, HbCO2) and the human body's skin surface flowing blood in oxygenation-deoxidization conditions and revealed the characteristic of OD value in the imaginable and unimaginable visible spectrum domain and this gave a foundation for the technology to be applied in the vitiligo sickness epidermis melanin pellet examination. METHODS: Using different spectrum and in vitro and in vivo methods, the authors did statistics for the information of OD value under different state and wavelength. RESULTS: Examination of in vitro: Experimented rabbit's blood Hb. O2 and RBC. O2 both have 367, 414 nm (the Soret cingulum) and 541, 576 (the Q cingulum) nm absorption peaks in the visible domain and both have 432 nm (Soret cingulum) and 553 nm (Q cingulum) absorption peaks, but blood had no change in the absorption peak position. No matter under what RBC and Hb condition, there was only completely independent absorption peak under the acidification and deoxidization condition. There is a significant difference (p < 0.01) between OD values under conditions of blood RBC cell and Hb hemolysis. Examination of in vivo: By using back skin surface specimen of the in vivo hand, absorption peaks were found at 540 and 576 nm for RBC. O2 condition and at 555 and 755 nm for RBC. CO2. Having selected the specimen of hand back skin (a: Nail, b: refers to skin, c: hand back skin), wavelengths were examined for the three dots. Among them, 545 nm absorption peak has average OD values of absorbency, which are 0.83 +/- 0.001, 0.73 +/- 0.001 and 0.62 +/- 0.001, and differences are notable (p < 0.01). CONCLUSION: Each absorption peak position of in vitro examination for RBC and Hb is invariable, but OD value of absorbency is different. Examination results under RBC condition are close to the aboriginality of RBC in vivo blood cell organization. The in vivo examination does not show any attack and damage to the human body, its sensitivity is high, testing time is short, and it has the superiority of taking in-phase test for the wavelength and the position information and so on. It is hopeful for the direct examination of epidermis black element and colored pellet.