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Studies on the Phenylfluorone-Mo(Ⅵ) Complex as Interacting Mode Spectroscopic Probe of Protein in OP Microemulsion Medium
作者姓名:Qin WEI  Dan WU  Bin DU  Qing Yu OUSchool of Chemistry & Chemical Engineering  Jinan University  Jinan 250022  Lanzhou Institute of Chemical Physics  Chinese Academy of Science  Lanzhou 730000
作者单位:Qin WEI,Dan WU,Bin DU,Qing Yu OUSchool of Chemistry & Chemical Engineering,Jinan University,Jinan 250022,Lanzhou Institute of Chemical Physics,Chinese Academy of Science,Lanzhou 730000
摘    要:The application of phenylfluorone (PF)-Mo(VI) complex as a spectroscopic probe is studied. In the presence of OP microemulsion at pH 3.04, PF-Mo(Ⅵ) complex combines protein rapidly to form a stable compound and the absorbance at 527 nm is in proportion to the concentration of protein in the range 0-16 μg mL-1 for bovine serum albumin (BSA). OP microemuslion media is introduced into protein determination, it has increased markedly the sensitivity of the system. The molar absorption coefficient was 5.98×l06 L mol-1 cm-1 for BSA. The assay, with sensitivity, simplicity and tolerance to many foreign substances, is applied to the determination of protein in samples with satisfactory results. Moreover, the binding number of BSA with the complex, which is determined by molar ratio and slope ratio methods, is in good agreement.

关 键 词:Protein   spectrophotometry   PF-Mo(Ⅵ) complex   microemulsion   enhanced sensitivity.
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