Time-resolved optical Kerr-effect spectroscopy of low-frequency dynamics in Di-L-alanine,poly-L-alanine,and lysozyme in solution |
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Authors: | Giraud Gerard Wynne Klaas |
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Affiliation: | Department of Physics, University of Strathclyde, Glasgow G4 0NG, Scotland. |
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Abstract: | The low-frequency spectra of peptides and proteins in solution have been investigated with optical heterodyne-detected Raman-induced Kerr-effect spectroscopy. Spectra were obtained for di-l-alanine ALA(2) and poly-l-alanine (PLA) in dichloroacetic acid solution. The conformational dependence of those spectra at low frequency has been analyzed. ALA(2) displays a band centered at 50 cm-1, whereas the alpha-helical PLA shows two shoulders at 60 and 140 cm-1. The similarity of the spectral features observed in PLA to those in water can be explained by analogous acoustic translational modes in the hydrogen network of the PLA alpha-helix. The mostly alpha-helical protein lysozyme in aqueous solution has also been investigated and showed significantly more structure with modes at 10, 35, 73, 106, and 164 cm-1. |
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