Energetics and dynamics of peptide fragmentation from multiple-collision activation and surface-induced dissociation studies |
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Authors: | Laskin Julia |
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Affiliation: | Fundamental Science Directorate, Pacific Northwest National Laboratory, PO Box 999 (K8-88), Richland, WA 99352, USA. Julia.Laskin@pnl.gov |
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Abstract: | This account summarizes the energetics and dynamics of peptide fragmentation obtained using a new approach recently developed in our laboratory. The approach involves RRKM modeling of time- and energy- resolved tandem mass spectrometry (MS/MS) data obtained using collisional activation. We demonstrate that surface-induced dissociation (SID) on a long time-scale of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) is perfectly suited for studying the energetics and dynamics of peptide fragmentation. The advantages provided by SID include very fast ion activation, which eliminates possible discrimination against higher-energy dissociation pathways, and efficient "amplification" of small changes in dissociation parameters. We present a summary of results obtained for small alanine-containing peptides as well as larger peptides including angiotensin analogs and a series of peptides containing the LDIFSDF motif. |
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