Simultaneous determination of retinol and tocopherols by high-performance liquid chromatography. |
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| Authors: | Y Satomura M Kimura Y Itokawa |
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| Institution: | Department of Hygiene, Faculty of Medicine, Kyoto University, Japan. |
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| Abstract: | A high-performance liquid chromatographic (HPLC) method to determine retinol and all four tocopherols (alpha-, beta-, gamma- and delta-) simultaneously was established using a reversed-phase column (YMC-PACK A-302 S-5 120A ODS). The HPLC conditions were mobile phase 65% isopropanol, sample solvent 99.5% methanol and temperature 30 degrees C. Retinol and tocopherols were measured in rat liver. |
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