Lipase immobilized on poly(VP-co-HEMA) hydrogel for esterification reaction |
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Authors: | Mahiran Basri Sunana Samsudin Mansor Bin Ahmad Che Nyonya Abdul Razak Abu Bakar Salleh |
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Institution: | (1) Center for Research in Enzyme and Microbial Technology, del Jabatan Kimi, Fakulti Sains and Pengajian Alam Sekitar, Universiti Putra Malaysia, 43400 Serdang, Malaysia |
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Abstract: | Lipase from Candida rugosa was immobilized by entrapment on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate)(polyVP-co-HEMA]) hydrogel, and divinylbenzene was the crosslinking agent.
The immobilized enzymes were used in the esterification reaction of oleic acid and butanol in hexane. The activities of the
immobilized enzymes and the leaching ability of the enzyme from the support with respect to the different compositions of
the hydrogels were investigated. The thermal, solvent, and storage stability of the immobilized lipases was also determined.
Increasing the percentage of composition of VP from 0 to 90, which corresponds to the increase in the hydrophilicity of the
hydrogels, increased the activity of the immobilized enzyme. Lipase immobilized on VP(%):HEMA(%) 90∶10 exhibited the highest
activity. Lipase immobilized on VP(%):HEMA(%) 50∶50 showed the highest thermal, solvent, storage, and operational stability
compared to lipase immobilized on other compositions of hydrogels as well as the native lipase. |
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Keywords: | Lipase immobilization hydrogel esterification stability |
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