Supercritical carbon dioxide extraction of Usnea longissima (L.) Ach.: Optimization by Box-Behnken design (BBD) |
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Authors: | Ceren ATİ LA Dİ NÇ ER,Ceren GÖ KALP,Bengü GETİ REN,Atila YILDIZ,Nuray YILDIZ |
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Affiliation: | 1. Department of Chemical Engineering, Faculty of Engineering, Ankara University, Tandoğan, Ankara, Turkey ; 2. Department of Biology, Ankara University, Ankara, Turkey |
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Abstract: | Usnic acid (UA) was extracted from Usnea longissima (L.) Ach. in supercritical carbon dioxide (SC-CO2) medium. The selected process parameters were extraction temperature (35–45 °C), amount of co-solvent (0%–5%) and extraction time (5–9 h). These parameters were applied to Box-Behnken design (BBD) belonging to response surface methodology (RSM) to determine optimum process parameters for the highest amount of UA in the extract. g UA/100g lichen, extraction yield % and UA content values were calculated in the range of 0.045–0.317, 2.77–5.4 and 71%–82% in different experimental conditions, respectively. The optimum conditions were predicted as 42 °C, 4.3% (ethanol) and 7.48 h. It was determined that the predicted and experimental values of g UA/100g lichen were compatible, and the suggested model was valid. |
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Keywords: | Usnea longissima (L.) Ach. usnic acid (UA) supercritical extraction 9response surface methodology (RSM) |
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