Application of matrix solid-phase dispersion to the determination of amitrole and urazole residues in apples by capillary electrophoresis with electrochemical detection |
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Authors: | M. Moreno E. Bermejo A. Sánchez M. Chicharro A. Zapardiel |
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Affiliation: | (1) Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain;(2) Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia, 28040 Madrid, Spain |
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Abstract: | A new method based on matrix solid-phase dispersion (MSPD) extraction was studied for the extraction of amitrole (3-amino-1,2,4-triazole), and its metabolite urazole (3,5-dihydroxy-1,2,4-triazole), in apple samples. The influence of experimental conditions on the yield of the extraction process and on the efficiency of the cleanup step was evaluated. Determination was carried out by capillary electrophoresis (CE) with electrochemical detection, demonstrating the compatibility between MSPD and CE techniques. The method has been successfully applied to different apple varieties. Recoveries in samples spiked at 1.6 and 1.7 μg g−1 for amitrole and urazole were 88 and 82%, respectively. The limits of detection were 0.4 μg g−1 for both compounds using electrochemical detection. |
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Keywords: | Matrix solid-phase dispersion Capillary electrophoresis Electrochemical detection Amitrole Urazole |
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