ENERGY TRANSFER IN TRIMERIC C-PHYCOCYANIN STUDIED BY PICOSECOND FLUORESCENCE KINETICS |
| |
Authors: | J. Wendler W. John H. Scheer A. R. Hoezwarth |
| |
Affiliation: | Max-Planck-Institut für Strahlenchemie, Stiftstr.34–36, D–4330 Mülhcim a.d. Ruhr and 2Botanisches Institut der Universität, Menzingerstr. 67, D–8000 München 19, W. Germany |
| |
Abstract: | Abstract— The excited state kinetics of trimeric C-phycocyanin from Mastigocladus laminosus has been measured as a function of the emission and excitation wavelength by the single-photon timing technique with picosecond resolution and simultaneous data analysis. A fast decay component of 22 ps (C-phycocyanin with linker peptides) and 36 ps (C-phycocyanin lacking linker peptides) is attributed to efficient energy transfer from sensitizing to fluorescing chromophores. At long detection wavelengths the fast decay components are found to turn into a rise term. This finding further corroborates the concept of intramolecular energy transfer. Previous reports on the conformational heterogeneity of the chromophores and/or proteins in C-phycocyanin are confirmed. Our data also provide indications for the importance of the uncoloured linker peptides for this heterogeneity. |
| |
Keywords: | |
|
|