| 新颖的双胰岛素取代钴(III)卟啉的制备和表征 |
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| 引用本文： | 潘瑞花,张天乐,韩定献,刘长林. 新颖的双胰岛素取代钴(III)卟啉的制备和表征[J]. 中国化学, 2006, 24(8): 1037-1044. DOI: 10.1002/cjoc.200690193 |
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| 作者姓名： | 潘瑞花 张天乐 韩定献 刘长林 |
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| 作者单位： | Department of Chemistry Huazhong University of Science and Technology，Wuhan，Hubei 430074，China，Department of Chemistry，Huazhong University of Science and Technology，Wuhan，Hubei 430074，China，Department of Chemistry，Huazhong University of Science and Technology，Wuhan，Hubei 430074，China，Department of Chemistry，Huazhong University of Science and Technology，Wuhan，Hubei 430074，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China(No.20571028). |
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| 摘 要： | A novel insulin-disubstituted Co(Ⅲ)protoporphyrin IX,CoPI,where I is insulin and CoP is Co(Ⅲ)protopor-phyrin IX,was prepared by covalently coupling the propionate groups on the porphyrin ring to the ε-amino groupsof B29-Lys on insulin via amide linkages.The FTIR spectra in the amide I region(1600—1700 cm~(-1))and circulardichroism study show that CoPI has a conformation similar to the insulin at pH 6.9,whereas it exhibits significantconformational changes in structure as compared with the insulin self at pH 8.2.The pH absorption titration indi-cates that the alkaline conditions(pH≥8.0)are required for the formation of complexes between the free CoP andthe insulin.The thermodynamic and kinetic data reveal that free CoP is bound to either the zinc-insulin or free insu-lin with a dissociation constant of(2.0±0.3)×10~(-5)or(2.2±0.3)×10~(-5)mol/L.
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| 关 键 词： | 金属卟啉 胰岛素 共价化合物 制备 表征 Co（Ⅲ） 钴 |
| 收稿时间： | 2005-08-30 |
| 修稿时间： | 2005-08-302006-04-12 |
Preparation and Characterization of a Novel Insulin‐Disubstituted Co(III) Porphyrin |
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| PAN Rui-Hua, ZHANG Tian-Le, HAN Ding-Xian, LIU Chang-Lin. Preparation and Characterization of a Novel Insulin‐Disubstituted Co(III) Porphyrin[J]. Chinese Journal of Chemistry, 2006, 24(8): 1037-1044. DOI: 10.1002/cjoc.200690193 |
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| Authors: | PAN Rui-Hua ZHANG Tian-Le HAN Ding-Xian LIU Chang-Lin |
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| Abstract: | A novel insulin‐disubstituted Co(III) protoporphyrin IX, CoPI, where I is insulin and CoP is Co(III) protoporphyrin IX, was prepared by covalently coupling the propionate groups on the porphyrin ring to the ε‐amino groups of B29‐Lys on insulin via amide linkages. The FTIR spectra in the amide I region (1600–1700 cm?1) and circular dichroism study show that CoPI has a conformation similar to the insulin at pH 6.9, whereas it exhibits significant conformational changes in structure as compared with the insulin self at pH 8.2. The pH absorption titration indicates that the alkaline conditions (pH≧8.0) are required for the formation of complexes between the free CoP and the insulin. The thermodynamic and kinetic data reveal that free CoP is bound to either the zinc‐insulin or free insulin with a dissociation constant of (2.0±0.3)×10?5 or (2.2±0.3)×10?5 mol/L. |
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| Keywords: | metalloporphyrin insulin covalent attachment |
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