高效液相色谱-串联质谱法同时测定烟草中5种Amadori化合物

建立了高效液相色谱-三重四极杆质谱（HPLC-QqQ MS）联用定量分析烟草中5种Amadori前香物质的方法。样品经含5%（v/v）甲醇的水提取后，采用Waters XBridge^TM Amide色谱柱（250 mm×4.6 mm，3.5 μm）、以甲醇-水为流动相进行分离，采用多反应监测（MRM）模式测定烟草样品中5种Amadori化合物的含量。结果表明，5种Amadori化合物的峰面积与质量浓度的线性关系良好，相关系数r为0.9895~0.9989；定量限（S/N=10）在10.18~44.58 μg/L，检出限（S/N=3）在3.51~14.86 μg/L；平均加标回收率为92.6%~123.6%，相对标准偏差（RSD）小于9.4%。本方法操作简便、灵敏度高、分析速度快，可以用于烟叶及卷烟中5种主要Amadori类物质的含量测定。

Simultaneous determination of five Amadori compounds in tobacco by high performance liquid chromatography-mass spectrometry

A method of high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QqQ MS) in multiple reaction monitoring (MRM) mode for simultaneous determination of five Amadori compounds in tobacco has been developed. The separation was performed on an XBridge^TM Amide column (250 mm×4.6 mm, 3.5 μm) by gradient elution using methanol and water as mobile phases. The linearity of this method was good with the correlation coefficients (r) of 0.9895-0.9989. The limits of detection (S/N=3) were from 3.51 μg/L to 14.86 μg/L, and the limits of quantitation (S/N=10) were from 10.18 μg/L to 44.58 μg/L. The recoveries varied from 92.6% to 123.6% with the relative standard deviations (RSDs) less than 9.4%. The established method is rapid, accurate, robust and convenient, and it could be applied to the measurement of the five Amadori compounds in tobacco and cigarettes.