液相色谱-同位素比质谱法同时测定葡萄酒中甘油和乙醇的δ13C值

采用液相色谱-同位素比质谱(LC-IRMS)技术建立了同时测定葡萄酒中甘油和乙醇δ13C值的分析方法。优化了葡萄酒中影响甘油和乙醇色谱分离的条件。方法的精密度和准确度分别为0.15‰~0.26‰和0.11‰~0.28‰。对40个葡萄酒样品进行了测定,甘油和乙醇的δ13 C值分别为-26.87‰~-32.96‰、-24.06‰~-28.29‰,两者具有较强的相关性(R=0.82)。该方法不需要复杂的样品预处理,在相同条件下同时测定甘油和乙醇的δ13C值,较传统方法简单、快速。

Simultaneous determination of δ13C values of glycerol and ethanol in wine by liquid chromatography coupled with isotope ratio mass spectrometry

A novel procedure was established for the characterization of δ¹³C values of glycerol and ethanol in wine by liquid chromatography-isotope ratio mass spectrometry (LC-IRMS). Several parameters influencing the separation of glycerol and ethanol from wine matrix were optimized. The precision and accuracy of the proposed method were 0.15‰ to 0.26‰ and 0.11‰ to 0.28‰, respectively. The results obtained for 40 wine samples displayed that the δ¹³C value of glycerol ranged from -26.87‰ to -32.96‰ and that of ethanol ranged from -24.06‰ to -28.29‰. Close correlations (R=0.82) were obtained between the δ¹³C values of glycerol and ethanol. The proposed method didn''t need complex sample treatment, and the δ¹³C values of glycerol and ethanol in wine can be simultaneously determined, thus improving the method in terms of simplicity and speed compared with traditional methods.