通过柱切换技术与高效液相色谱联用的限进性柱对盐酸贝那普利的在线富集性能

以自制的限进性填料柱为预处理富集柱,Luna C₁₈柱为分析柱,通过柱切换技术将限进性填料柱与高效液相色谱联用(RAM-HPLC),研究了盐酸贝那普利的在线富集效果。考察了进样体积与峰面积、系统总压力的关系,以及常规进样与大体积进样的差别。当进样体积在100 μL以内时,峰面积随进样体积的增加而增加;当进样体积大于80 μL时,系统总压力变化明显。考虑对整个系统的保护,选择80 μL作为最大进样体积。同一浓度的样品进样20 μL与进样80 μL所得峰面积之间的线性关系良好。RAM柱对盐酸贝那普利具有良好的富集作用,能够有效提高HPLC的灵敏度,而且具有简单、经济的特点。

Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride

The online enrichment ability of the restricted-access media (RAM) column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride in plasma was studied. The RAM-HPLC system consisted of an RAM column as enrichment column and a C18 column as analytical column coupled via the column switching technique. The effects of the injection volume on the peak area and the systematic pressure were studied. When the injection volume was less than 100 μL, the peak area increased with the increase of the injection volume. However, when the injection volume was more than 80 μL, the pressure of whole system increased obviously. In order to protect the whole system, 80 μL was chosen as the maximum injection volume. The peak areas of ordinary injection and the large volume injection showed a good linear relationship. The enrichment ability of RAM-HPLC system was satisfactory. The system was successfully used for the separation and detection of the trace benazepril hydrochloride in rat plasma after its administration. The sensitivity of HPLC can be improved by RAM pre-enrichment. It is a simple and economic measurement method.