Statistical mixture design selective extraction of compounds with antioxidant activity and total polyphenol content from Trichilia catigua |
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Authors: | Audrey Alesandra Stinghen Garcia Lonni Renata Longhini Gisely Cristiny Lopes João Carlos Palazzo de Mello Ieda Spacino Scarminio |
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Institution: | 1. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, BR-87020-900, Maringá, PR, Brazil;2. Laboratório de Quimiometria em Ciências Naturais, Departamento de Química, Universidade Estadual de Londrina, Caixa Postal 6001, BR-86051-970, Londrina, PR, Brazil |
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Abstract: | Statistical design mixtures of water, methanol, acetone and ethanol were used to extract material from Trichilia catigua (Meliaceae) barks to study the effects of different solvents and their mixtures on its yield, total polyphenol content and antioxidant activity. The experimental results and their response surface models showed that quaternary mixtures with approximately equal proportions of all four solvents provided the highest yields, total polyphenol contents and antioxidant activities of the crude extracts followed by ternary design mixtures. Principal component and hierarchical clustering analysis of the HPLC–DAD spectra of the chromatographic peaks of 1:1:1:1 water–methanol–acetone–ethanol mixture extracts indicate the presence of cinchonains, gallic acid derivatives, natural polyphenols, flavanoids, catechins, and epicatechins. |
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Keywords: | Catuaba Extraction optimization Mixture modeling Simplex-centroid design Trichilia catigua barks |
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